長時間的緊迫反應除了影響動物的行為和生理作用外，也可能會導致較高的死亡率，因此了解動物之緊迫因子有其重要性。群居性靈長類複雜的社會關係和互動，個體之性別和年齡之生理特性，以及所面對的自然環境下食物資源和氣候變化等因子都可能會影響個體之緊迫程度。本研究以非侵入性(non- invasive)的研究方式，進行已知個體排遺之皮質醇 (cortisol)濃度分析，檢視野生台灣獼猴(Macaca cyclopis)糞腎上腺皮質醇濃度與位階、年齡、性別、生殖狀態和季節之關係。研究自2009年1月至2010年1月在宜蘭福山試驗林進行特定猴群已知個體之排遺樣本收集，期間共收集391個新鮮排遺樣本，樣本以化學冷光免疫法 (Chemiluminescent immunoassay, CLIA)進行皮質醇濃度檢測。所測得之糞腎上腺皮質醇濃度範圍為 1.55～49.7ng/g(mean±sd:10±0.54ng/g, n=391)，同一個體於一天中不同時段所收集之樣本的皮質醇濃度無顯著差異，沾到尿液樣本與無尿液樣本的皮質醇濃度亦無顯著差異。猴群內個體全年之糞腎上腺皮質醇濃度呈季節變化，在春季及夏季之皮質醇濃度顯著較高，但春季和夏季間，以及秋季和冬季間的皮質醇濃度則無顯著差異。個體之生殖狀態、性別、年齡和位階等特性與其糞腎上腺皮質醇濃度間無顯著相關性。
Chronic stress could affect behavior, physiology of animals and cause higher mortality, therefore it is important to understand the stressors of animals. It has been suggested that group-living primates may exhibit elevated stress level due to environmental change, food scarcity, as well as social relationship and interactions with their groupmates. The stress level is also related to age and sex of the animal. In this study, fecal cortisol of wild Taiwanese macaques(Macaca cyclopis)was analyzed to investigate how social dominance rank, kinship, reproductive status, and environmental factors affect the stress level of individuals in different age/sex classes. A total of 391 fresh fecal samples of known individuals in a macaque group were collected during January 2009 and January 2010 in the Fushan experimental forest, on which the cortisol levels were examined by the Chemiluminescent immunoassay(CLIA). The range of fecal cortisol appeared from 1.55 to 49.7ng/g(mean±sd:10±0.54ng/g, n=391). Fecal samples differing in the collecting time of the day and urine content were not significantly different in the cortisol level. No effect of age or sex on the fecal cortisol level was found. The fecal cortisol level appeared to show seasonal variation. It was significantly higher in spring and summer than in fall and winter. There was no significant association between the fecal cortisol level and the reproductive status, kinship, and social dominance rank in the Taiwanese macaque.