北台灣寵物及其畜主之金黃色葡萄球菌腸毒素之分子流行病學研究

外文標題: 
Molecular Epidemiology of Enterotoxins of Staphylococcus aureus from Pets and Their Owners in Northern Taiwan
校院系所: 
國立台灣大學 獸醫學系研究所
指導教授: 
周晉澄
出版年份: 
2010年
主題類別: 
摘要: 

本研究針對台灣北部地區(台北市、台北縣、桃園縣),無金黃色葡萄球菌感染臨床症狀之寵物及其畜主之鼻腔以棉棒採樣,分離出金黃色葡萄球菌並檢測其腸毒素之基因型,並使用逆向被動乳膠凝集法(reverse passive latex agglutination, RPLA)檢測其傳統型腸毒素A、B、C、D、E之表現型,及使用限制酵素SmaI切割細菌之DNA後,進行脈衝式膠體電泳(Pulse-field gel electrophoresis, PFGE)分析菌株之相關性,並且作抗生素感受性試驗,以了解不同腸毒素基因型和抗藥性與其脈衝式膠體電泳分析分析結果之關係。結果共分離到116株金黃色葡萄球菌,之中有74株(63.8%)至少帶有一種腸毒素基因,最常被偵測到的腸毒素基因型為sea、seb、sek、sep、seq、egc等,另外,採集於人的分離株(95株)帶有腸毒素基因之比例(69.5%),顯著高於分離自寵物(狗與貓)之分離株(21株)(38.1%)(P

外文摘要: 

In order to investigate the Staphylococcus aureus enterotoxin genotypes and phylogenetic relatedness, samples were collected with nasal swab from pet and their owners and using Pulse-field gel electrophoresis (PFGE) patterns of these isolates to analyze their phylogenetic relatedness with enterotoxin genotypes and antimicrobial susceptibility. The commercial RPLA test kit SET-RPLA detecting enterotoxins A, B, C, D, and E was available for this study to compare with the strain genotypes. The results of our previous sampling show that the strains of methicillin-resistant Staphylococcus aureus (MRSA) have a higher percentage (91.3%) of harboring enterotoxin genes than the methicillin-sensitive S. aureus (MSSA) do (57%) (P