母貓中性化之後之葡萄糖耐受性試驗

外文標題: 
The Effect of Intravenous Glucose Tolerance Test in Neutralized Female Cats
校院系所: 
中興大學 獸醫學系暨研究所
指導教授: 
李衛民
出版年份: 
2009年
主題類別: 
摘要: 

在1991年,Crane等人首先發表了貓隻中性化與肥胖的相關性,攝食量的上升和代謝率下降是中性化後肥胖的主要原因。在人和貓,肥胖都會導致胰島素阻抗和葡萄糖耐受性異常,而胰島素阻抗和葡萄糖耐受性異常則被認為是第二型(非胰島素依賴型)糖尿病發生的前置因子。本實驗目的在觀察母貓中性化後六個月內的體重、胰島素敏感性和葡萄糖耐受性的變化,藉此觀察母貓中性化與糖尿病之間的關係。本實驗使用四隻三歲齡未中性中性化的母貓,中性化後每天測量其體重並計算BMI,在中性化後第一至六個月,每個月進行一次靜脈內葡萄糖耐受性試驗(intravenous glucose tolerance test),採血前先置入頭靜脈留置針,採血的時間點為葡萄糖注射前和葡萄糖注射後10、20、30、60及120分鐘,血中胰島素和葡萄糖分別以商品化之Mercodia Feline Insulin ELISA和ACCU-CHECK® Active血糖機進行檢測,胰島素敏感性以禁食後胰島素和HOMA值進行觀察,其值越高則胰島素敏感性越低,HOMA值之計算方法為禁食後血糖乘以禁食後胰島素濃度除以22.5,葡萄糖耐受性則以曲線下面積進行觀察。結果可見體重和BMI在中性化後第二個月顯著高於中性化後初始之時 (p

外文摘要: 

In 1991, it was first reported that obesity in feline was associated with neutering. Increased food intake and decreased metabolic rate after neutering might be the reason leading to obesity. Obesity will lead to insulin resistance and impaired glucose tolerance in human and feline. Type 2 diabetes mellitus (non-insulin dependent diabetes mellitus) is preceded by insulin resistance and impaired glucose tolerance. The aim of this study is to investigate the relationship between neutralization and diabetes mellitus in female cats. In our study, four 3-year-old female neutered cats were enrolled into this observation. Body weight of cats was measured every day. Intravenous glucose tolerance test was performed at 1, 2, 3, 4 and 5 months after neutering. Blood samples were collected at 0 and 10, 20, 30, 60 and 120 minutes from intravenous catheter after glucose administration. Plasma insulin and glucose were measured by Mercodia Feline Insluin ELISA and ACCU-CHECK® Active, respectively.In the results, body weight and BMI of cats were significantly higher at 2, 3, 4 and 5 months after neutering in comparison to the time after neutering (p